AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE
نویسندگان
چکیده
منابع مشابه
Autoradiography with the Electron Microscope
Autoradiographic techniques for use with the electron microscope have been developed and considerably improved in recent years. A review of this historical development is given by Meek and Moses (14), and a theoretical analysis of the problems involved by Pelc (17) and Caro (4). In this paper, we describe a method for preparing autoradiographic specimens, using a new, fine-grained emulsion and ...
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A technic is described for high resolution intracellular autoradiography in the electron microscope. Cultures of LLC-MK(2) monkey kidney cells were incubated for 72 hours in a medium containing 0.4 microcurie per ml of thymidine-H(3). After labeling, the cells were fixed with osmium tetroxide and embedded in methacrylate. Ultrathin sections of the labeled tissue were taken up on Formvar-coated ...
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The "circle analysis" method of Williams (1969) and a new improved method employing hypothetical grains described in the previous paper have been used to analyze the distribution of autoradiographic grains over erythroid bone marrow cells labeled with radioactive iron, (55)Fe. The resolution obtainable with this isotope was determined by measuring the distribution of grains about a thin line so...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1964
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.22.2.469